Journal: Frontiers in Behavioral Neuroscience

Article Title: In depth behavioral phenotyping unravels complex motor disturbances in Cstb −/− mouse, a model for progressive myoclonus epilepsy type 1

doi: 10.3389/fnbeh.2023.1325051

Figure Lengend Snippet: Timeline of the behavioral tests. Each animal completed all behavioral tests, except for Phenotyper that was performed only for a subset of animals: Phenotyper—myoclonus detection; CatWalk—walking pattern (coordination), speed of walk; Startle—startle response, prepulse inhibition; Beam walk—coordination; Elevated plus maze—hyperactivity, speed of walk; Grip strength—strength.

Article Snippet: Startle response and prepulse inhibition (PPI) tests were performed using Startle Response (TSE Systems) operating on Startle Response/PPI Version 03.00 software.

Techniques: Inhibition

Journal: Frontiers in Behavioral Neuroscience

Article Title: In depth behavioral phenotyping unravels complex motor disturbances in Cstb −/− mouse, a model for progressive myoclonus epilepsy type 1

doi: 10.3389/fnbeh.2023.1325051

Figure Lengend Snippet: Cstb −/− mice show progressive myoclonus and altered startle response. (A) Myoclonic events were detected in PhenoTyper cages for a period of 3 h at 1.5, 3, and 6 months of age. (B) Progression of myoclonic events from 1.5 to 6 months of age in individual animals (see for separate graphs of Cstb −/− mice). (C) Cstb −/− mice show reduced startle response to 100 dB noise at 3, 5, and 6 months of age compared to the wild type ( wt ) mice. (D–F) Prepulse inhibition (PPI) at 69, 73, and 77 dB prepulse intensities is decreased in Cstb −/− mice at the age of 3 (D) , 5 (E) , and 6 (F) months. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001, N = 12 for (A,B) , N = 15–16 for (C–F) .

Article Snippet: Startle response and prepulse inhibition (PPI) tests were performed using Startle Response (TSE Systems) operating on Startle Response/PPI Version 03.00 software.

Techniques: Inhibition

Journal: Frontiers in Behavioral Neuroscience

Article Title: In depth behavioral phenotyping unravels complex motor disturbances in Cstb −/− mouse, a model for progressive myoclonus epilepsy type 1

doi: 10.3389/fnbeh.2023.1325051

Figure Lengend Snippet: Comparison of the symptoms in EPM1 patients and in Cstb −/− mice.

Article Snippet: Startle response and prepulse inhibition (PPI) tests were performed using Startle Response (TSE Systems) operating on Startle Response/PPI Version 03.00 software.

Techniques: Comparison, Inhibition

Journal: Progress in neuro-psychopharmacology & biological psychiatry

Article Title: HU-910, a CB2 receptor agonist, reverses behavioral changes in pharmacological rodent models for schizophrenia.

doi: 10.1016/j.pnpbp.2022.110553

Figure Lengend Snippet: Fig. 3. Effects of HU-910 pretreatment on MK-801 and amphetamine-induced PPI disruption. (A) The disruptive effect of MK-801 treatment (0.25 mg/kg) in the PPI was attenuated by HU-910 (30 mg/kg) in all prepulse intensities. *p < 0.05 compared to vehicle+saline group (n = 9/group). (B) The startle response amplitude (pulse-only trials, arbitrary units, AU) was not modified by any treatment (C). Effect of HU-910 pretreatment on amphetamine-induced PPI disruption. The disruptive effect of amphetamine treatment (5 mg/kg) in the PPI was attenuated by HU-910 treatment at all doses tested on 80 dB prepulse intensity. On 85 dB and 90 dB prepulse intensities, HU-910 pretreatment attenuated PPI disruption only ate the dose of 3 mg/kg *p < 0.05 compared to vehicle+saline group (n = 10/group). (D) The startle response amplitude (pulse-only trials, arbitrary units, AU) was not modified by any treatment. Repeated-measures ANOVA followed by Turkey's test.

Article Snippet: The prepulse inhibition (PPI) test was performed simultaneously on two identical startle response systems (Med Associates, USA) with a constant 65 dB background noise.

Techniques: Disruption, Saline, Modification

Journal: Brain, Behavior, & Immunity - Health

Article Title: A20/TNFAIP3 heterozygosity predisposes to behavioral symptoms in a mouse model for neuropsychiatric lupus

doi: 10.1016/j.bbih.2019.100018

Figure Lengend Snippet: Fig. 3. Behavioral phenotypes observed in A20þ/ female mice aggravate after immune challenge. (A) Overall spontaneous activity and; (B) activity during the dark period of female A20þ/ mice are significantly lower in comparison to wildtype littermates; (C) A20þ/ female mice display an anxiety-related phenotype as is evidenced from the open field experiment where they spent significantly less time in the center and; (D) more time in the periphery in comparison to wildtype littermates; (E) A normal startle response was observed in both genotypes, however; (F) A20þ/ female animals showed significantly higher startle reactivity when a prepulse was preceding the original startle tone. This is indicative for a sensorimotor gating impairment. Data are represented as meanSEMs and statistical differences were determined using repeated-measures two-way ANOVA using Dunnett’s multiple comparisons test for post hoc analysis (A, F), unpaired t-test (B, C, E), Mann Whitney test (D) and two-way ANOVA using Sidak’s multiple comparisons test for post hoc analysis (F); (*P < 0.05, **P < 0.01).

Article Snippet: Disease-associated phenotypes: Startle reactivity and prepulse inhibition (PPI) of the acoustic startle reflex were measured using a Med Associates acoustic startle box (St. Albans, VT, USA) as described previously (Naert et al., 2011b).

Techniques: Activity Assay, Comparison, MANN-WHITNEY